Yui Makino (Sketchbook ~full color'S~'s Hazuki, Tsubasa: RESERVoir CHRoNiCLE's Sakura) as Francesca, the heroine of the story. She's an "undead idol" who's a little spacey, but has a lot of energy. Francesca is a zombie girl with a big appetite, and her favorite foods are dishes from Hokkaido.

Asami Tano (Smile Precure!'s Cure Sunny, Toriko's Rin) as Exorcist, a girl who is always chasing after Francesca and always gets the short end of the stick because she does so. As Francesca does not pay attention to her much, she is unhappy, but considers the zombie idol her rival. However, she has problems dealing with Francesca's dog, Franken.

Hiroki Takahashi (Yu-Gi-Oh!'s Jonouchi Katsuya, Hetalia - Axis Powers's Japan) as Toshizo Hijikata, the demonic captain of the Shinsengumi. He is very stubborn, and will never be disloyal. He also is good at looking after his subordinates, and is respected because of that. Unfortunately, he also is a bit in love with himself.

Tarusuke Shingaki (Fate/Zero's Kariya, Ghost in the Shell Arise's Togusa) as Kazue Soma, a man who is a member of the Shinsengumi who gives orders to the troops when Hijikata is not around, but never listens to what other says. Despite this, he is quite the worldly-wise man.

Daisuke Nagumo as Nobori Nakajima, a member of the Shinsengumi. He is very clumsy, but also knowledgeable. He raises flowers as a hobby.

Ami Naito as Tetsunosuke Ichimura, a member of the Shinsengumi and Hijikata's favorite page. Being only 14 1/2 years old, his personality is determined and mischievous.

Katsuya Miyamoto as Kai Shimada, a member of the Shinesengumi. He's a giant man with a giant sweet tooth to boot. Until his death, he participated in a majority of the battles with the Shinsengumi.