1. Opening Licks

"I Love Rock & Roll" - Joan Jett & The Blackhearts
"I Wanna Be Sedated" - The Ramones
"Thunder Kiss '65" - White Zombie
"Smoke on the Water" - Deep Purple
"Infected" - Bad Religion

2. Axe-Grinders

"Iron Man" - Black Sabbath
"More Than a Feeling" - Boston
"You've Got Another Thing Comin'" - Judas Priest
"Take Me Out" - Franz Ferdinand
"Sharp Dressed Man" - ZZ Top

3. Thrash And Burn

"Killer Queen" - Queen
"Hey You" - The Exies
"Stellar" - Incubus
"Heart Full of Black" - Burning Brides
"Symphony of Destruction" - Megadeth

4. Return of the Shred

"Ziggy Stardust" - David Bowie
"Fat Lip" - Sum 41
"Cochise" - Audioslave
"Take It Off" - The Donnas
"Unsung" - Helmet

5. Fret-Burners

"Spanish Castle Magic" - Jimi Hendrix
"Higher Ground" - Red Hot Chili Peppers
"No One Knows" - Queens of the Stone Age
"Ace Of Spades" - Motörhead
"Crossroads" - Cream

6. Face-Melters

"Godzilla" - Blue Öyster Cult
"Texas Flood" - Stevie Ray Vaughan
"Frankenstein" - The Edgar Winter Group
"Cowboys from Hell" - Pantera
"Bark at the Moon" - Ozzy Osbourne

Bonus Tracks

"Fire It Up" - Black Label Society
"Cheat on the Church" - Graveyard BBQ (Pemenang "Be a Guitar Hero" Kontestan)
"Caveman Rejoice" - The Bags
"Eureka, I've Found Love" - The Upper Crust
"All of This" - Shaimus
"Behind The Mask" - Anarchy Club
"The Breaking Wheel" - Artillery
"Callout" - The Acro-brats
"Decontrol" - Drist
"Even Rats" - The Slip
"Farewell Myth" - Made in Mexico
"Fly on the Wall" - Din
"Get Ready 2 Rokk" - Freezepop
"Guitar Hero" - Monkey Steals The Peach
"Hey" - Honest Bob and the Factory-to-Dealer Incentives
"Sail Your Ship By" - Count Zero
"Story of My Love" - The Model Sons

Axel Steel (Classic Metal)
Clive Winston (Classic Rock/Psychedelic Rock)
Judy Nails (Alternative Rock)
Johnny Napalm (Punk Rock)
Pandora (Nu Metal)
Xavier Stone (Prog-Rock)

Ada 2 karakter yang bisa didapatkan:

Izzy Sparks (Hair Metal, Glam Metal)
Grim Ripper (Death Metal)

id.wikipedia.org/wiki/Guitar_Hero