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Shirayuki is a bright and resourceful girl, determined, able to speak her mind, and knowledgeable in both botany and medicine. Whenever there is a problem or conflict, she often takes matters into her own hands. She later learns to rely on others, especially Zen.
Due to her rare hair color, Shirayuki gets a proposal to be a concubine of the foolish but famous prince Raji. As she cannot decline it, she escapes to the neighboring country. While traveling, she meets a boy named Zen, who accidentally gets injured, and she boldly cures his wounds. They become friends, and she finds out than Zen is actually a prince of that country, and he saves her from being taken by Raji. They later go to his palace, where she applies for the job of 'Palace Pharmacist', to be able to go freely to the castle where Zen is.
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Working for Zen, Obi, is a strange (but lighthearted mostly) and unusual character in the story. His first appearance is when he tries to frighten Shirayuki by shooting an arrow to a wall next to her. He tends to hide his face under a mask, which makes many people think of him as a bad guy. He later starts showing to have feelings for Shirayuki, for she is the only person that understands him. Out of all the characters in AnS, he is the only character with unknown motives for following Zen so far which makes him a rather mysterious character.
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The 2nd prince of the Kingdom of Clarines. He is in love with Shirayuki and has told his brother, Izana that he wishes to make her his wife. Zen is an excellent swordsman and has a penchant for exiting out windows instead of doors and jumping down from high places. As a young child, Zen has built up an immunity against various poisons by exposing himself to poisons in specific controlled doses causing convulsions, fever, and difficulty walking to name a few. These results were carefully documented by his doctors.
